From the journals: JLR
Determining how lipid pathways and cardiovascular disease are linked is a complex and rapidly evolving quest. Read about recent findings published in the Journal of Lipid Research.
Furin as a metabolic deactivator of PCSK9
The enzyme proprotein convertase subtilisin/kexin type 9, or PCSK9, regulates cholesterol metabolism by binding and chaperoning the low-density lipoprotein, or LDL, receptor to be degraded. That receptor clears LDL from circulation, making PCSK9 an appealing drug target: Blocking PCSK9 lowers blood LDL concentration, thereby reducing risk for cardiovascular disease.
PCSK9 has two known active forms: a mature form and a less understood furin-cleaved form, PCSK9_55. Carlota Oleaga and colleagues at the Oregon Health & Science University recently published the results of their studies to characterize this less known form in the Journal of Lipid Research.
First, Oleaga's team transfected a line of human embryonic kidney cells with the mature form of PCSK9 and furin vectors to test where PCSK9_55 was formed. As expected, the mature form was furin-cleaved to form PCSK9_55, with greater amounts of PCSK9_55 found in the extracellular space and only moderate amounts within cells. Adding a furin inhibitor that could cross the cell membrane decreased this extracellular formation.
The researchers engineered the embryonic kidney cells to express a recombinant PCSK9_55. Lacking a prodomain sequence that is essential to PCSK9 secretion, this form's abundant intracellular expression and retention were unsurprising. However, no change in secretion was detected when the recombinant PCSK9_55 was expressed with a prodomain or a prodomain donor. These results suggest that PCSK9_55 is not secreted into the extracellular space because it is markedly altered from the uncleaved parent.
Oleaga and colleagues propose that furin could act as a metabolic deactivator of PCSK9. Furin post-translationally modifies extracellular PCSK9 to form PCSK9_55, which has reduced capacity for LDL receptor degradation. When PCSK9_55 reenters the cell, it cannot be secreted back into circulation and eventually is catabolized. Further testing of this model could provide insight into drug development and treatments for cardiovascular disease.
Expressing CETP slows endotoxin clearance in mice
When Gram-negative bacteria enter the bloodstream, they release lipopolysaccharides, or LPSs, which activate the innate immune system. LPSs are not metabolized in the body and must be disaggregated and excreted in bile through a pathway known as the reverse LPS transport, or RLT, pathway. Increasing our knowledge of the RLT pathway could help with detection and treatment of sepsis, a life-threatening inflammatory immune response to infection that affects over 1.7 million adults in America each year.
Aloïs Dusuel and colleagues at the University of Burgundy recently published findings in the Journal of Lipid Research that a protein tightly related to key players of the RLT pathway, cholesteryl ester transfer protein, or CETP, does not contribute to the binding and transfer of LPS in plasma. However, when the researchers injected purified LPS into mice to simulate infection, the mice that were genetically altered to express CETP cleared endotoxins at a slower rate than mice who expressed no CETP. The CETP-expressing mice also had worse sepsis outcomes. The researchers suggest looking into the inflammatory response over time to better understand the role CETP plays in the inflammatory process and, possibly, in sepsis.
A look at atherosclerotic lesions in diabetes
Atherosclerosis is a progressive condition that increases risk of heart disease. Jenny Kanter and Karin Bornfeldt of the University of Washington School of Medicine have shown previously that the low insulin levels characteristic of diabetes lead to increased levels of apolipoprotein C3, or APOC3, and triglyceride-rich lipoproteins, or TRLs, as well as accelerated atherosclerosis.
Kanter and Bornfeldt recently published fluorescence microscopy images of atherosclerotic lesions in a mouse with induced Type 1 diabetes in the Journal of Lipid Research. Their experiment focuses on two apolipoproteins: APOB, which is the primary apolipoprotein of low-density lipoprotein, very low-density lipoprotein, chylomicrons and remnant lipoproteins; and APOC3, which slows the clearance of triglyceride-rich lipoproteins and their remnant lipoprotein particles, or RLPs, lipids that have been linked previously to cardiovascular disease.
The researchers found that APOC3 and APOB colocalize in atherosclerotic lesions of the aorta, supporting their hypothesis that the lipoproteins containing APOC3 particles could be these TRLs and RLPs. Their images also show that APOC3 and APOB are localized near lesional macrophages. Accumulation of these TRLs and RLPs can lead to increased accumulation of lesional macrophages, thereby worsening atherosclerosis and increasing cardiovascular disease risk.
Join the ASBMB Today mailing list
Sign up to get updates on articles, interviews and events.
As scientists argue whether a previous bout of covid offers the same amount of protection as vaccinations, people turn to the courts to decide.