Airola learns from failure

You can’t say Michael Airola wasn’t warned.

While trying to develop a research program that would differentiate his own lab from his postdoctoral mentor’s, he set out to determine the structure of phospholipase D.

Michael Airola

“I cautioned Mike that there was an elephant graveyard of grad students and postdocs who had attempted to solve these structures,” Michael Frohman, who had discovered the protein 20 years prior, wrote in a recommendation letter.

But Airola, who received the ASBMB’s 2022 Walter A. Shaw Young Investigator Award in Lipid Research, said, “I was optimistic … I thought, ‘we have a chance; we had an interesting idea, and I don’t think anyone has tried that, so we should try it.’”

That idea was to remove a section from the middle of the protein that appeared, based on its amino acid sequence, to have high intrinsic disorder. The more orderly portions left behind crystallized beautifully.

When it became clear that the approach would work, Airola said, he told graduate student Forrest Bowling, “It looks like you may have a successful Ph.D. project.” Bowling, he recalled, answered, “What do you mean, this ‘could be’ a Ph.D. project? Why do you think I joined your lab?”

Airola knew that not every graduate student answers their first research question — especially if it depends on crystallizing a protein. He had started his own dissertation interested in the structure of circadian signaling in human cells. “Through failure, I learned quite a bit,” he said.

He transitioned to a question about bacterial chemotaxis; studying a transmembrane signaling system got him interested in membrane biology, which brought him to Yusuf Hannun’s lab at Stony Brook University. It was a lasting match. As a postdoc, Airola determined the structure of two sphingolipid-metabolizing enzymes. Later, he landed a job as a professor at Stony Brook. Both Hannun and his wife and collaborator, the late Lina Obeid, were supportive and influential mentors, Airola said.

Airola’s own recent collaborations to boost graduate students and postdocs have included co-organizing a weekly virtual seminar series in lipid research that as many as 500 researchers attended at the height of pandemic lockdowns.