Journal News

A mold’s dangerous responses to its environment

Laurel Oldach
Jan. 19, 2021

Aflatoxins are among the most dangerous of natural products. At a high dose, the toxins can cause fatal liver failure; at lower doses, by forming adducts with guanine bases in DNA, they can cause mutations that lead to liver cancer.

Mold-445x334.jpg
Janice Haney Carr and Robert Simmons
A scanning electron micrograph shows the fruiting body of an Aspergillus fungus.

The toxins are made by filamentous fungi in the Aspergillus family found in soil and are able to colonize the grains and seeds that constitute many of the world's most important food crops. Aspergilli don't need aflatoxins to survive; they activate aflatoxin synthesis in response to environmental conditions, especially heat and moisture. Since hotter days are coming worldwide, researchers would like to find strategies to reduce aflatoxin production.

The genome of Aspergillus flavus, the chief culprit in introducing aflatoxin to human and animal food supplies, first was sequenced in 2006. But there's a difference between knowing what sequences are in a genome and knowing what they do; many sections of the A. flavus genome have not been annotated, meaning that researchers have had little insight into their function.

In a recent article in the journal Molecular & Cellular Proteomics, researchers at the Fujian Agriculture and Forestry University in China, led by Mingkun Yang, report on a proteogenomic analysis of A. flavus. By using the whole fungal genome instead of only its known coding sequences as the reference database to identify peptides detected through mass spectrometry, the team discovered over 700 new protein-coding genes.

"The authors provide a significant improvement to the genome annotation in Aspergillus and demonstrate the use of proteogenomics as a tool especially in organisms lacking high-quality genome annotations," one anonymous peer reviewer wrote.

Researchers cultured the fungus under cold, salty and oxidative stress conditions to maximize phenotypic variability, and they were rewarded: The fungi expressed a smorgasbord of proteoforms, including over 200 new-to-science splice variants, some single-amino-acid variants and a few unexpected intergenic peptides. In follow-up quantitative PCR experiments, the researchers observed that stressful conditions substantially affected the expression of some of the new genes.

Based on homology to other, better-annotated proteins in the literature, the authors think that they may have identified new metabolic enzymes, signaling proteins and stress response factors. They have not yet determined whether any of the new genes are involved in aflatoxin production.

According to the researchers, follow-up studies of the new protein-coding genes and when and where they are expressed may improve our understanding of when and why aflatoxin is produced.

Enjoy reading ASBMB Today?

Become a member to receive the print edition monthly and the digital edition weekly.

Learn more
Laurel Oldach

Laurel Oldach is a former science writer for the ASBMB.

Get the latest from ASBMB Today

Enter your email address, and we’ll send you a weekly email with recent articles, interviews and more.

Latest in Science

Science highlights or most popular articles

‘Inert’ ingredients in pesticides may be more toxic to bees than scientists thought
News

‘Inert’ ingredients in pesticides may be more toxic to bees than scientists thought

Dec. 9, 2023

Pesticide users sometimes know very little about how inerts function. That’s partly because they are regulated very differently than active ingredients.

FDA approves CRISPR–Cas9 therapy for sickle cell disease
News

FDA approves CRISPR–Cas9 therapy for sickle cell disease

Dec. 8, 2023

The gene editing treatment from Vertex Pharmaceuticals and CRISPR Therapeutics is the first of its kind

From the journals: MCP
Journal News

From the journals: MCP

Dec. 8, 2023

Metals contaminate protein samples. Lipid droplets go haywire in Huntington’s. Nerve cells respond to stimulation. Read about these recent papers.

The perfect storm
Feature

The perfect storm

Dec. 6, 2023

The world has 2023 Nobel laureates Katalin Karikó, Drew Weissman and others to thank for laying a foundation for the COVID-19 vaccine decades before the pandemic.

Throw your hat in the ring!
Annual Meeting

Throw your hat in the ring!

Dec. 6, 2023

Apply to speak at Discover BMB in Chicago in 2025.

How bacteria inhibit gene expression
Journal News

How bacteria inhibit gene expression

Dec. 5, 2023

Researchers show how altering activity of the transcription factor CarD can cause downregulation and upregulation of some genes.