Hippocampal lipids linked to brain disorders
The hippocampus is a fundamental brain region for memory processes, and its function is impaired early in the onset of neurodegenerative disorders such as Alzheimer’s disease, or AD.
Most studies addressing the hippocampus have considered it as a whole structure, but it also can be divided into subregions along its longitudinal axis, segregating dorsal and ventral poles. For instance, the dorsal hippocampus, or DH, contributes differentially to specific dimensions of spatial memory, while the ventral hippocampus, or VH, is proposed to be implicated predominantly in behaviors linked to emotions.
Lipids are major brain constituents, so we performed mass spectrometry lipidomic analysis of hippocampal subregions along the longitudinal axis. Within the lipidomic signatures we uncovered, we observed that the DH presents increased levels of phosphatidic acid and decreased levels of phosphatidylcholine compared to the VH, potentially implicating the phospholipase D, or PLD, pathway in DH–VH axis regulation.
Although six PLDs are found in mammals, only PLD1 and PLD2 have reported canonical PLD activity, which is based on the hydrolysis of phosphatidylcholine in the presence of water to free choline and phosphatidic acid, a known second messenger signaling lipid. Using mice that were genetically altered to lack Pld1 and Pld2 genes, we gathered data to support the hypothesis that PLD1 and PLD2 are the only contributors to PLD activity in the mouse forebrain.
PLD1 ablation significantly decreased the hippocampal levels of phosphatidic acid, affecting predominantly the DH lipidome, but upon PLD2 ablation, only minor lipid changes occurred, including increased PLD products suggesting PLD1 upregulation.
Since PLD1 was the main PLD activity source, we then focused predominantly on characterizing the effects of PLD1 ablation and showed that the mice lacking Pld1 presented specific deficits in novel object recognition and social interaction, disruption in dendritic arborization, and altered synaptic plasticity in the DH. Overall, we determined that PLD1 ablation impairs hippocampal functioning, predominantly affecting the DH, which, due to its allocated functions, is predicted to be particularly affected in Alzheimer’s disease.
We previously had observed that PLD2 ablation is protective in mice genetically altered to have Alzheimer’s. Future studies should address cross-regulation mechanisms between PLD1 and PLD2 and how these can be used to develop therapeutic strategies to treat or prevent hippocampal dysfunction and memory deficits.
Want more lipid research news?
Check out Lipid Trends, a curated collection of hot picks from the world of lipid research, brought to you by LIPID MAPS.
Enjoy reading ASBMB Today?
Become a member to receive the print edition monthly and the digital edition weekly.
Learn moreGet the latest from ASBMB Today
Enter your email address, and we’ll send you a weekly email with recent articles, interviews and more.
Latest in Science
Science highlights or most popular articles
Immune cells can adapt to invading pathogens
A team of bioengineers studies how T cells decide whether to fight now or prepare for the next battle.
Hinton lab maps structure of mitochondria at different life stages
An international team determines the differences in the 3D morphology of mitochondria and cristae, their inner membrane folds, in brown adipose tissue.
National Academies propose initiative to sequence all RNA molecules
Unlocking the epitranscriptome could transform health, medicine, agriculture, energy and national security.
From the journals: JLR
What can you do with artificial lipoproteins? A new key to angiogenesis. Flavonoids counteract oxidative stress. Read about recent papers on these topics.
Iron could be key to treating a global parasitic disease
A study has found that leishmaniasis causes body-wide changes in iron balance, leading to red blood cell damage.
Environmental DNA is everywhere
The ability to extract trace bits of DNA from soil, water, and even air is revolutionizing science. Are there pitfalls?