Journal News

Harnessing a natural plant insecticide for commercial use

Elisabeth Adkins Marnik
June 25, 2024

Many plants produce molecules or peptides that help them combat destructive insects. These natural insecticides hold a lot of promise for improving how we can protect crops around the world from pests without harming the environment 

Developing plant insecticides into eco-friendly products is one area of interest in David Craik’s laboratory, which is part of the Institute for Molecular Bioscience at the University of Queensland, Brisbane, Queensland, Australia.

Craik’s lab identified a group of cyclotides in a prior study and found that they have natural insecticidal properties. Cyclotides are unique; unlike typical peptides that form linear chains of amino acids, cyclotides link together end to end to form circular peptides. These cyclopeptides can interact with specific phospholipids that form cell membranes. This can happen within the insect’s gut and cause cell swelling or lysis that results in death or growth restriction of the insect. The Craik lab has become interested in studying the applications of these cyclotides, particularly as potential pesticides.

In their recent study, conducted in collaboration with Syngenta Crop Protection AG, an international agriculture technology company, and published in the Journal of Biological Chemistry, Craik’s lab focused on a specific cyclotide known as kalata B1. For an insecticide to have broad use, a business must be able to manufacture it efficiently and cost-effectively. It must also remain stable in a variety of environmental conditions where factors such as temperature and pH may differ considerably.

To address these issues, the Craik lab mutated specific amino acids within the cyclotide to determine their impact on the molecule's stability under varying conditions. This was achieved through a technique called a lysine scan, where each amino acid within the cyclotide was systematically replaced with lysine. The researchers then evaluated whether the change enhanced the stability of the cyclopeptide.

This work enabled the lab to create a more stable cyclopeptide capable of targeting insect cells from the fall armyworm, a major pest worldwide. They also confirmed that they could manufacture this cyclopeptide efficiently and safely.

“We can actually use a natural recombinant process to produce these proteins,” Craik said. “Some types of peptide synthesis can be quite an environmentally unfriendly process in terms of the amount of waste materials produced. But if you can produce peptides, and then cyclize them using enzymes rather than chemicals, you have a much greater production pathway.”

As of now, the only other cyclotide insecticide on the market is one known as Sero-x. Approved for use in Australia, it is applied to cotton plants and certain vegetable crops.

The next step for the Craik lab is to feed the cyclotide insecticide to fall armyworms and determine if it is effective in killing them. If these trials prove successful, another cyclotide insecticide may soon be available for use.

Enjoy reading ASBMB Today?

Become a member to receive the print edition four times a year and the digital edition monthly.

Learn more
Elisabeth Adkins Marnik

Elisabeth Adkins Marnik is the science education and outreach coordinator at the MDI Biological Laboratory in Bar Harbor, Maine, where she is spearheading the development of new programming.  This work is driven by her passion for making science accessible to students and the public. She is an ASBMB Today volunteer contributor as well as a contributing writer to Those Nerdy Girls and The Global Autoimmune Institute. Follow her on Instagram @sciencewhizliz.
 

Related articles

From the journals: JBC
Ken Farabaugh
From the Journals: JBC
Emily Ulrich
From the journals: JBC
Emily Ulrich
From the Journals: JBC
Ken Farabaugh
From the journals: JBC
Ken Farabaugh

Get the latest from ASBMB Today

Enter your email address, and we’ll send you a weekly email with recent articles, interviews and more.

Latest in Science

Science highlights or most popular articles

New mass spectrometry tool accurately identifies bacteria
Journal News

New mass spectrometry tool accurately identifies bacteria

April 30, 2025

Scientists develop a software tool to categorize microbe species and antibiotic resistance markers to aid clinical and environmental research. Read about this recent article published in Molecular & Cellular Proteomics.

New tool matches microbial and metabolic metaproteomic data
Journal News

New tool matches microbial and metabolic metaproteomic data

April 30, 2025

Scientists develop a bioinformatics program that maps omics data to metabolic pathways. Read about this recent article published in Molecular & Cellular Proteomics

Meet Paul Shapiro
Interview

Meet Paul Shapiro

April 29, 2025

Learn how the JBC associate editor went from milking cows on a dairy farm to analyzing kinases in the lab.

CRISPR epigenome editor offers potential gene therapies
News

CRISPR epigenome editor offers potential gene therapies

April 25, 2025

Scientists from the University of California, Berkeley, created a system to modify the methylation patterns in neurons. They presented their findings at ASBMB 2025.

Finding a symphony among complex molecules
Profile

Finding a symphony among complex molecules

April 23, 2025

MOSAIC scholar Stanna Dorn uses total synthesis to recreate rare bacterial natural products with potential therapeutic applications.

E-cigarettes drive irreversible lung damage via free radicals
Journal News

E-cigarettes drive irreversible lung damage via free radicals

April 17, 2025

E-cigarettes are often thought to be safer because they lack many of the carcinogens found in tobacco cigarettes. However, scientists recently found that exposure to e-cigarette vapor can cause severe, irreversible lung damage.