Chronic fatty liver disease
Nonalcoholic fatty liver disease, or NAFLD, and its progressive form, nonalcoholic steatohepatitis, or NASH, have emerged as significant public health concerns in Western societies. NAFLD is a continuum of chronic fatty liver diseases ranging from benign hepatic steatosis to NASH, which consists of fatty liver with inflammation and injury. NASH can progress to severe fibrosis or cirrhosis, and primary hepatocellular cancer, or HCC. The increase in NAFLD in adults and children over the last 20 years parallels the obesity epidemic in Western societies. Factors contributing to the increased incidence of NAFLD include a sedentary lifestyle and poor diet of fat, simple sugar and cholesterol.
IMAGE COURTESY OF DONALD B. JUMP
Since there are no U.S. Food and Drug Administration-approved drugs for NASH treatment, current therapies rely on lifestyle modification and treatment of the comorbidities associated with NAFLD, including obesity, hyperglycemia, dyslipidemia, hypertension and type 2 diabetes. Several clinical studies have evaluatedomega-3 fatty acids in NAFLD therapy, because omega-3 fatty acids have been reported to augment fatty-acid oxidation and triglyceride catabolism and suppress fatty-acid synthesis, inflammation and blood levels of triglycerides. Moreover, humans and mice with NAFLD have low hepatic omega-3 and omega-6polyunsaturated fatty acid, or PUFA content, when compared with healthy individuals (See Depner, C. M. et al 2013 and Arendt, B. M. et al 2015).
Most clinical studies use a mix of eicosapentaenoic acid (20:5 EPA) and docosahexaenoic acid (22:6 DHA) and report that these dietary lipidslower liver fat but do not reduce liver fibrosis, a risk factor for cirrhosis and HCC. In contrast, NAFLD patients consuming EPA have no reduction in hepatic fat or fibrosis. Studies of mice with NASH that lack the low-density lipoprotein receptor, or LDLR -/- have shown that DHA, but not EPA, reduces Western dietinduced fatty liver, inflammation and fibrosis (See Depner, C. M. et al 2013 and Lytle, K. A. et al 2015).
The differential action of C20–22 omega-3 fatty acids on clinical outcomes can be explained, at least in part, by effects on fatty-acid metabolism and differences in the molecular actions of EPA versus DHA. DHA and EPA inhibit fatty-acid synthesis by suppressing the nuclear abundance of sterol regulatory element binding protein-1, a transcription factor controlling the expression of enzymes involved in de novo lipogenesis and PUFA synthesis. As such, humans and mice consuming EPA alone show no significant increase in blood or hepatic DHA content (See Depner, C. M. et al 2013 and Itakura, H. M. et al 2011). DHA consumption, however, increases blood and hepatic DHA, EPA and the longchain n-3 fatty acid docosapentaenoic acid, or DPA. EPA and DPA increase through retroconversion.
Dietary DHA, but not EPA, attenuates Western diet-induced nuclear accumulation of transcription factors involved in inflammation, such as NF-kappa B, and fibrosis, such as phospho-Smad3. While NF-kappa B controls the expression of multiple inflammatory factors, including Cox2,chemokines and cytokines, phospho-Smad3 is a downstream mediator of TGF-beta signaling. TGF-beta is a major regulator of hepatic stellate cellfunction and fibrosis. The impact of DHA on mouse liver fibrosis is TGF-beta receptors, factors regulating TGF-beta signaling, collagensubtypes, and enzymes involved in protein crosslinking and extracellular matrix remodeling.
Together, these studies establish that DHA controls several transcriptional regulatory networks relevant to NAFLD. There remain, however, several unanswered questions. Chief among these is determining why the mix of EPA and DHA fails to affect hepatic fibrosis in humans significantly. It will require more investigation to understand how omega-3 PUFA control pathways linked to chronic fatty liver disease.
Join the ASBMB Today mailing list
Sign up to get updates on articles, interviews and events.
Severe scrutiny of two major papers, including one about the effectiveness of hydroxychloroquine, is part of science's normal process of self-correction, explains Mark R. O’Brian.
As COVID-19 shuttered laboratories across the U.S., many researchers were forced to euthanize the animals they study. Lindsay Gray, a rodent surgeon in an animal research lab that faced this dilemma, argues here there is a safer, more effective way.
Early in adulthood, the brain regenerates lost myelin effectively, but remyelination falters with age. Researchers seek to understand why — and what the change may mean for people with multiple sclerosis.
Nearly 6 million Americans live with Alzheimer’s disease without solid treatment options.