MCP: A spotlight on reproductive proteomics
In a collection of articles highlighting recent discoveries in sperm and egg biology, a special issue of the journal Molecular & Cellular Proteomics celebrates the contribution of the field of proteomics to a deeper understanding of reproductive biology.
The Molecular & Cellular Proteomics special issue cover by artist Julie Newdoll illustrates the diversity of reproductive systems in the animal kingdom.Julie Newdoll
Edited by MCP Associate Editor Tim Karr of the Biodesign Institute at Arizona State University, the issue showcases the versatility and multiplicity of proteomic technologies for analysis of protein abundance, post-translational modifications, protein–protein interactions and subcellular localization in reproduction.
According to Karr, proteomic approaches are particularly well-suited to studying reproductive biology.
“Sexual reproduction in many cases can be thought of as cellular action at a distance, that is, sperm travels outside the body in which it was produced,” Karr said. “Therefore, we can expect that a majority of interactions and interesting biology takes place predominantly at the protein–protein interaction level.”
Sperm are known to transmit packaged RNAs, but compared with somatic cells, sperm are transcriptionally inactive, further highlighting the importance of protein interactions.
Proteomics is also important in the study of interactions between the male ejaculate and the female reproductive tract. There is little experimental evidence that gene expression or other gene–gene interactions play a major role once the ejaculate is delivered to the reproductive tract.
“For these cases, proteomics is a fruitful approach if one wishes to understand the molecular mechanisms involved,” Karr said.
The special issue dives into a few major themes in reproductive biology. A series of papers investigates how proteomes of reproductive cells change throughout the reproductive processes, such as spermatogenesis, sperm maturation and capacitation, and oocyte activation.
Human fertility also is explored in depth in this special issue. A few studies delineate the proteomes of infertile sperm and sperm that undergo different abstinence periods. Another study provides the first draft map of the human ovarian proteome and its extracellular matrix, which can inform the development of artificial ovaries and provide greater understanding of fertility in women.
Two papers seek to understand polyandry, a pattern of mating in which a female animal has more than one male mate. One study investigates how polyandry drives sexual selection, and another looks to understand the protein–protein interactions that mediate sperm competition dynamics and preservation.
The special issue includes studies in various organisms, from humans to insects and even crocodiles. The diversity of organisms highlights the importance of studying reproduction. One study discovered that crocodile sperm undergo capacitation, a functional maturation that was thought to be restricted to mammals. The fact that an analogous process was observed in a distant reptilian species could challenge what we know about the evolution of species.
“Sperm and egg are the only cell types for which their function is universal throughout the animal kingdom,” Karr said. “From an evolutionary viewpoint, sperm and egg are the glue that binds life, and therefore their constituent proteomes may represent our deepest links to the evolution of eukaryotic life on the planet.”
Enjoy reading ASBMB Today?
Become a member to receive the print edition four times a year and the digital edition monthly.
Learn moreGet the latest from ASBMB Today
Enter your email address, and we’ll send you a weekly email with recent articles, interviews and more.
Latest in Science
Science highlights or most popular articles

New mass spectrometry tool accurately identifies bacteria
Scientists develop a software tool to categorize microbe species and antibiotic resistance markers to aid clinical and environmental research. Read about this recent article published in Molecular & Cellular Proteomics.

New tool matches microbial and metabolic metaproteomic data
Scientists develop a bioinformatics program that maps omics data to metabolic pathways. Read about this recent article published in Molecular & Cellular Proteomics

Meet Paul Shapiro
Learn how the JBC associate editor went from milking cows on a dairy farm to analyzing kinases in the lab.

CRISPR epigenome editor offers potential gene therapies
Scientists from the University of California, Berkeley, created a system to modify the methylation patterns in neurons. They presented their findings at ASBMB 2025.

Finding a symphony among complex molecules
MOSAIC scholar Stanna Dorn uses total synthesis to recreate rare bacterial natural products with potential therapeutic applications.

E-cigarettes drive irreversible lung damage via free radicals
E-cigarettes are often thought to be safer because they lack many of the carcinogens found in tobacco cigarettes. However, scientists recently found that exposure to e-cigarette vapor can cause severe, irreversible lung damage.