Award

Tabor award
for bacterial colony work

Catherine Back honored for studies of oral pathogen that infects heart
Laurel Oldach
March 01, 2018

Why can a bacterium usually found in the mouth also drive an infection linked to heart failure? How does a nascent bacterial colony in the heart protect itself from being swept away? Catherine Back won a 2018 Journal of Biological Chemistry/Herbert Tabor Young Investigator Award for her work on the mechanism of adhesion between a bacterial fibril protein and human tissues.

Catherine Back found a novel mechanism for streptococcal adhesion to fibronectin. Courtesy of Catherine Back

The protein, which she characterized while she was a postdoctoral fellow at the University of Bristol, helps the oral bacterial species Streptococcus gordonii attach to and colonize human tissues and may contribute to bacterial infection of the heart.

Back’s first research experience was as an undergraduate at Bristol, where she worked with microbiologist Howard Jenkinson on an interaction between S. gordonii and another species of commensal oral bacteria. She stayed on at Bristol for her Ph.D., extending her undergraduate studies with a closer and more multidisciplinary look at CshA, an adhesion protein.

“I thought it was really interesting to work on a protein that not much was known about,” she said.

While Jenkinson remained her primary supervisor, for her project, Back drew on the expertise of three principal investigators from two departments. Jenkinson and Angela Nobbs were frequent collaborators on microbiology research in the dental school, while biochemist Paul Race contributed expertise in protein characterization.

“I managed to work in both departments, learning microbiology and also structural biology,” Back explained. The collaboration between the groups continues to this day. The labs now work together to characterize other bacterial adhesins.

After earning her Ph.D., Back stayed on in Bristol to finish the work described in her JBC article and then moved to the nearby University of Bath to study a new type of vaccine for tuberculosis.

Back grew up in Exeter in Devon, England, and said she enjoys getting outdoors both in Bristol and on visits home. “I often go back to my parents’ house in Exeter,” she said. “It’s near the sea, and near Dartmoor, which is a really nice place to go hiking.”

Back recently returned to Bristol to join the lab of Paul Race for a new project, studying antimicrobials from bacteria that colonize deep-sea sponges.

Catching fibronectin to clamp on tightly

Adhesion proteins are key drivers of bacterial colony formation. In the case of S. gordonii, the protein CshA contributes to binding to the human extracellular glycoprotein fibronectin.

Because S. gordonii can enter the bloodstream and adhere to heart valves, the binding mechanism may have implications for development of heart infections.

Catherine Back and colleagues at the University of Bristol divided a relatively uncharacterized region of the protein into three domains through bioinformatic analyses, publishing their work in JBC in December 2016.

They analyzed each domain’s interaction with fibronectin, finding that one of the three domains did not interact. Of the remaining two, dubbed NR1 and NR2, the on and off rates for NR1 were faster, although NR2 was capable of higher affinity binding. They analyzed the structure of both domains, determining that both are responsible for fibronectin binding. The disordered NR1 domain interacts transiently with fibronectin, the so-called catch. After NR1 makes initial contact, NR2 binds more tightly — the clamp.

Back suspects the binding she described may be relevant to other pathogens. A number of other streptococci “have CshA-like proteins, which have a similar sequence and may have a similar mechanism of interaction,” she said.

Laurel Oldach

Laurel Oldach is a science writer for the ASBMB.

Join the ASBMB Today mailing list

Sign up to get updates on articles, interviews and events.

Latest in People

People highlights or most popular articles

Herbert Tabor 1918 – 2020
Retrospective

Herbert Tabor 1918 – 2020

September 24, 2020

F. Peter Guengerich remembers the contributions of the longtime editor of the Journal of Biological Chemistry who died in August.

ASBMB welcomes new members
Member News

ASBMB welcomes new members

September 21, 2020

The American Society for Biochemistry and Molecular biology welcomed more than 60 new members in April.

It all comes down to where we place our bets
Interview

It all comes down to where we place our bets

September 18, 2020

Mark Harpel works in a research unit at GlaxoSmithKline that helps choose the most promising targets for new drug development.

Understanding the impact of Type 1 diabetes susceptibility genes
Research Spotlight

Understanding the impact of Type 1 diabetes susceptibility genes

September 17, 2020

Starting in eighth grade, a series of mentors who saw something special in Sharifa Love–Rutledge helped her stay on the path to being a researcher — and becoming a mentor to others.

Cell biology, microbiology societies present awards
Member News

Cell biology, microbiology societies present awards

September 14, 2020

Honorees include ASBMB members Joann Trejo, James Olzmann, Steven Farber, Sue Wickner and Bernard Moss.

Virtual volunteering in the land of lakes and volcanoes
Outreach

Virtual volunteering in the land of lakes and volcanoes

September 09, 2020

Brandon Roy encourages other scientists to teach students in Nicaragua through the Outreach 360 program