Journal News

Unveiling an elusive microbial enzyme

Christian McDonald
By Christian McDonald
April 26, 2022

In the 1980s, scientists isolated a novel protein from the common airborne fungus Aspergillus fumigatus. Initial studies described this enzyme’s functions, but the gene encoding it remained unknown for 40 years until it recently was identified by researchers at the University of Tokyo. Graduate student Toma Kashima and collaborators published this work in the Journal of Biological Chemistry, providing an extensive biochemical characterization of this enzyme.

Researchers determined that FFase1 displays a hexameric shape. This ribbon structure displays a tetramer, demarcated by three distinct colors, which features unique sites on aFFase1.
Toma Kashima & Shinya Fushinobu
Researchers determined that aFFase1 displays a hexameric shape. This ribbon structure displays a tetramer, demarcated by three distinct colors, which features unique sites on aFFase1.

Prebiotics are nondigestible substances that can help shape specific microbial populations within the digestive tract. They are frequently dietary fibers from plant food sources but can include other compounds. Because mammals lack the enzymes required to break down the bonds within prebiotics, we rely on the bacteria that reside in our gut to do this work. Specifically, a group of enzymes in bacteria known as glycoside hydrolases, or GHs, play a significant role in this process. The University of Tokyo researchers became interested in these GH enzymes within the genome of Bifidobacterium dentium, a bacterium that colonizes the human intestine and oral cavity.

“We first noticed a hypothetical gene next to a GH32 protein and predicted this unknown gene must have a similar function,” said Shinya Fushinobu, corresponding author of this paper.

To begin teasing out the function of this region of the bacterial genome, Fushinobu’s team isolated the protein product of this gene and tested what substances it reacts with using thin-layer chromatography, a method that identifies components of a mixture and follows the progression of a reaction.

“The result was very surprising … with a specific shift in one spot that we did not expect to be there,” Fushinobu said.

The team discovered that this protein had two distinct enzymatic activities, acting as both an alpha-D-fructofuranosidase and an alpha-D-arabinofuranosidase. These two activities break down specific bonds in sugars, the former targeting bonds found only in cooked substances, such as sucrose caramel. Previously studied GH proteins break down b-linked molecules (which hold sugars together), but the researchers found that these two activities acted on a-linked sugars, an area that is not well understood.

“This activity was unexpected because a-bonds and b-bonds are totally different, and cleavage of a-D-fructofuranoside bonds by a GH protein is new,” Fushinobu said.

Following these initial findings and 40 years after its initial discovery, the researchers designated the protein isolated from Bifidobacteriumas aFFase1.

Fushinobu’s team advanced their investigation by determining that aFFase1 has a hexameric structure. They proceeded to mutagenize specific sites within the protein that were revealed to be responsible for binding and reacting with substrates. Collectively, the team assembled their data and proposed a mechanism for how aFFase1 converts particular sugars in foods we consume.

Although there are over 160 recognized GH families, Bifidobacterium’s aFFase1 does not resemble any known amino acid sequence. Its unique features have prompted the creation of a new glycoside hydrolase family, GH172. After elucidating aFFase1’s role in breaking down distinct a-linked bonds within sugars, this research team will explore further the metabolic systems in B. dentium that exploit aFFase1.

“We unexpectedly revisited a very old enzyme discovered by Japanese researchers in the 1980s and found very surprising results,” Fushinobu said. “We believe this new gene will be involved in important degradation systems that can be broadly used in commercial products and give us insights for our health and the bacterial communities in the human gut.”

Enjoy reading ASBMB Today?

Become a member to receive the print edition four times a year and the digital edition weekly.

Learn more
Christian McDonald
Christian McDonald

Christian McDonald is a Ph.D. candidate in microbiology and immunology at the University of Miami Miller School of Medicine.

Related articles

From the journals: JBC
Ken Farabaugh
From the journals: JBC
Isabel Casas
From the journals: JBC
Emily Ulrich
From the journals: JBC
Marissa Locke Rottinghaus

Get the latest from ASBMB Today

Enter your email address, and we’ll send you a weekly email with recent articles, interviews and more.

Latest in Science

Science highlights or most popular articles

Does a protein hold the key to Alzheimer’s?
Journal News

Does a protein hold the key to Alzheimer’s?

Dec. 10, 2024

Researchers in Maryland and Massachusetts team up to study how SORL1 promotes tau trafficking and seeding in cells that leads to the neurodegenerative disorder.

Cracking the recipe for perfect plant-based eggs
News

Cracking the recipe for perfect plant-based eggs

Dec. 8, 2024

It involves finding just the right proteins. With new ingredients and processes, the next generation of substitutes will be not just more egg-like, but potentially more nutritious.

MSU researchers leverage cryo-EM for decades-in-the-making breakthrough
News

MSU researchers leverage cryo-EM for decades-in-the-making breakthrough

Dec. 7, 2024

Lee Kroos and Ben Orlando have reported the first high-resolution experimentally determined structures of the intramembrane protease SpolVFB.

From the Journals: MCP
Journal News

From the Journals: MCP

Dec. 6, 2024

Rapid and precise SARS-CoV-2 detection using mass spec. Mapping brain changes from drug addiction. Decoding plant osmotic stress response. Read about recent MCP papers on these topics.

What seems dead may not be dead
Award

What seems dead may not be dead

Dec. 4, 2024

Vincent Tagliabracci will receive the Earl and Thressa Stadtman Distinguished Scientist Award at the ASBMB Annual Meeting, April 12–15 in Chicago.

'You can't afford to be 15 years behind the parasite'
Award

'You can't afford to be 15 years behind the parasite'

Dec. 3, 2024

David Fidock will receive the Alice and C.C. Wang Award in Molecular Parasitology at the 2025 ASBMB Annual Meeting, April 12–15 in Chicago.