ASBMB Annual Meeting

MCP session focuses on proximity-dependent biotinylation

Anne-Claude Gingras
By Anne-Claude Gingras
March 18, 2021

How proteins interact with one another and with other macromolecules, their organization in cells and how these associations are modulated following perturbations long have defined an important branch of the field of functional proteomics. In the past 10 years, approaches that enable the study of these connections in the context of live cells — and now live organisms — have revolutionized the role of proteomics in cell biology.

This session at the 2021 ASBMB Annual Meeting focuses on proximity-dependent labeling approaches, which allow for the covalent tagging of proteins that have come in close proximity to a bait protein (a protein of interest fused to an enzyme) in the context of living cells. Coupling of this strategy to mass spectrometric identification of the labeled proteins permits the characterization of cellular organization.

Since its first implementation to identify the components of the nuclear lamina in cultured cells, proximity-dependent biotinylation has contributed to better understanding of the composition of both membrane-bound and membraneless organelles, defined new signaling complex components, and illuminated the molecular mechanisms underlying several diseases. Proximity-dependent biotinylation methods, including APEX and BioID, still are developing, permitting new experimental modalities and answering increasingly complex questions.

The three presenters selected by the editorial leadership of Molecular & Cellular Proteomics are early-career researchers pushing the boundaries of proximity-dependent biotinylation methods and their applications.

Tess C. Branon, a postdoctoral fellow at the University of California, Berkeley, will present her work on the directed evolution of promiscuous biotin ligases for efficient proteomic mapping in vivo.

Geoffrey Hesketh, a postdoctoral fellow at the Lunenfeld–Tanenbaum Research Institute of Mount Sinai Hospital in Toronto, will describe how he used proximity-dependent sensors to reveal new intricacies in amino acid signaling.

Ilia Droujinine, a principal investigator and Scripps fellow at Scripps Research in California, will introduce new work exploring protein trafficking by in vivo tissue-specific labeling in Drosophila.

Anne-Claude Gingras
Anne-Claude Gingras

Anne-Claude Gingras is an investigator at the Lunenfeld–Tanenbaum Research Institute of Mount Sinai Hospital in Toronto and a deputy editor of the journal Molecular & Cellular Proteomics.

Join the ASBMB Today mailing list

Sign up to get updates on articles, interviews and events.

Latest in People

People highlights or most popular articles

Spriggs to start lab; Cejka joins EMBO
Member News

Spriggs to start lab; Cejka joins EMBO

Aug. 2, 2021

Awards, promotions, milestones and more. Find out what's going on in the lives of ASBMB members.

In memoriam: Gerhard Meissner
In Memoriam

In memoriam: Gerhard Meissner

Aug. 2, 2021

He was a professor at the University of North Carolina at Chapel Hill and an ASBMB member for more than 40 years.

Meet Stephanie Moon
Observance

Meet Stephanie Moon

Aug. 2, 2021

She studies messenger RNA regulation at the University of Michigan in Ann Arbor.

Journal of Biological Chemistry names new editor-in-chief
Journal News

Journal of Biological Chemistry names new editor-in-chief

July 30, 2021

Alex Toker, professor of pathology at the Beth Israel Deaconess Medical Center and Harvard Medical School, will begin his term Oct. 1.

Conaway named vice provost; Emr wins Shaw Prize; remembering Hartley
Member News

Conaway named vice provost; Emr wins Shaw Prize; remembering Hartley

July 26, 2021

Awards, promotions, milestones and more. Find out what's going on in the lives of ASBMB members.

Remembering Turk and Yamamoto
In Memoriam

Remembering Turk and Yamamoto

July 19, 2021

We look back on the lives of a mass spectrometry pioneer and member of the JBC editorial board and a researcher focused on enzymology and the biochemistry of lipids involved in inflammation.