Journal News

From the journals: MCP

Rajamani Selvam
By Rajamani Selvam
August 20, 2020

We offer summaries of recent papers in the journal Molecular & Cellular Proteomics.

 

A new role for an insulin receptor substrate

Insulin receptor substrate 2, or IRS2, is an adaptor molecule recruited by insulin receptors to facilitate insulin signaling. As such, IRS2 is implicated in diabetes mellitus and in breast and pancreatic cancers and neuroblastoma. IRS2 function is known to mediate signaling including lipid uptake and metabolism, and key checkpoints for its function are the ubiquitin ligases that can break down the proteins that help IRS2.

Though some researchers have reported ubiquitin ligases that target both IRS2 and other IRS molecules, none had reported ubiquitin ligases that specifically target IRS2. Sandhya Manohar and a team at Harvard Medical School addressed this question with a proteomic screen of anaphase-promoting complex/cyclosome, or APC/C, to identify potential substrates during the intermediate G1 growth phase of the cell cycle. The APC/C assists in the breakdown of proteins involved in the cell cycle, achieving this feat with the help of activators. In their recent paper in the journal Molecular & Cellular Proteomics, the authors provide evidence suggesting IRS2 is a direct substrate of APC/CCdh1, where Cdh1 is an APC/C co-activator during G1.

When APC/C was blocked and/or Cdh1 was knocked down, the researchers observed increased IRS2 levels. They also found that IRS2 depletion decreases the expression of cell-cycle regulatory proteins and perturbs the mitotic spindle checkpoint during the mitotic phase. The stabilization of IRS2 depends on a unique D-box motif present in IRS2, thereby establishing a new mode of regulating IRS2.

Together, these findings identify a ubiquitin ligase that targets only IRS2 and not the other main IRS protein, IRS1, and establish a new role for IRS2 as a cell-cycle checkpoint regulator. In addition to IRS2 roles in lipid uptake and metabolism, this study unravels a new role of IRS2 in cell-cycle regulation.

MCP-fig-FTJ-890x549.jpg
Sandhya Manohar et al./MCP
Insulin receptor substrate 2 primarily targets for proteasomal degradation. When anaphase-promoting complex/cyclosome is inactivated, IRS2 increases. This increases the expression of cell-cycle proteins, which is important for normal progression through the mitotic phase of the cell cycle.

Creating a neural signaling roadmap

Neurotransmitters transmit chemical signals from synapses to facilitate cell–cell communication. Impairments in synapse function play a key role in the pathology of neurological diseases such as Parkinson’s, schizophrenia and epilepsy. Synaptosomes, which are stable and functional nerve terminals and can be manipulated for various processes, are employed to understand molecular processes of communication. These processes include post-translational modifications, or PTMs, which are important for protein function.

In a recent paper in the journal Molecular & Cellular Proteomics, Inga Boll and a team from the University of Southern Denmark write about the role of a PTM, sialylation (the addition or removal of sialic acid), in N-linked glycopeptides in rat synaptosomes. The researchers have made a large-scale comprehensive map of sialic acid–containing glycosylation sites. Protein kinases, proteases and phosphatases carry these sialylated glycosylation sites.

Brief depolarization of the synaptosomes allowed the researchers to identify changes in site-specific sialylated and desialylated N-linked glycoproteins. These changes occurred on essential synaptic proteins such as ion channels, transporters and synaptic vesicle proteins and are as dynamic as post-translational phosphorylation. This study unravels sialylation, a novel modulator that alters the neurotransmitter release in response to depolarization. The researchers have identified the need to establish state-of-the-art techniques to study the implications of the molecular functions of sialylation.

What ionizing radiation does to proteins

X-rays, gamma rays and ultraviolet rays are useful for disinfecting instruments and in diagnostic medical exams, but high doses of such radiation can damage tissues and lead to cancer. Cell death due to the production of reactive oxygen species, or ROS, such as hydrogen peroxide and superoxide can cause tissue damage. Researchers know that, at the DNA level, cells employ several strategies to avoid and repair DNA damage due to oxidation. However, the mechanisms involved in repairing the DNA damage are relatively unknown.

In a recent paper in the journal Molecular & Cellular Proteomics, Steven T. Bruckbauer and colleagues at the University of Wisconsin describe performing mass spectrometry on Escherichia colicells that they had treated with ionizing radiation to identify the resulting oxidative modifications. The researchers determined that fewer than 10% of the proteins present in the organism were affected by radiation. The most common oxidative modification was hydroxylation, followed by dioxidation. Glyceraldehyde 3′-phosphate dehydrogenase, or GAPDH, is the primary target of oxidation by radiation.

Similar oxidation on GAPDH is also present in human breast carcinoma cells, suggesting that it is a critical player in oxidative damage from bacteria to eukarya. This work adds to our knowledge of the repertoire of cellular functions of GAPDH.

Rajamani Selvam
Rajamani Selvam

Rajamani Selvam is an Oak Ridge Institute for Science and Education research fellow at the U.S. Food and Drug Administration in Maryland. She is interested in science communication and policy.

Join the ASBMB Today mailing list

Sign up to get updates on articles, interviews and events.

Latest in Science

Science highlights or most popular articles

Rodents in space Keeping bone and muscle strong on the ISS
News

Rodents in space Keeping bone and muscle strong on the ISS

September 19, 2020

Researchers helped mice stay mighty with an experiment to counter the effects of microgravity. The gene treatment might also enhance muscle and bone health on Earth — and in humans.

Understanding the impact of Type 1 diabetes susceptibility genes
Research Spotlight

Understanding the impact of Type 1 diabetes susceptibility genes

September 17, 2020

Starting in eighth grade, a series of mentors who saw something special in Sharifa Love–Rutledge helped her stay on the path to being a researcher — and becoming a mentor to others.

Re-creating coagulation in a lab
Journal News

Re-creating coagulation in a lab

September 15, 2020

Threatened arthropods are in the crossfire of medical and conservation efforts, but new research could benefit horseshoe crabs and humans alike.

Decoy receptor neutralizes coronavirus in cell cultures
News

Decoy receptor neutralizes coronavirus in cell cultures

September 13, 2020

To keep COVID-19 from infecting tissues once they’re exposed, a new study led by Erik Procko suggests luring the virus with an engineered, free-floating receptor protein that binds the virus and blocks infection.

When plants and their microbes are not in sync, the results can be disastrous
News

When plants and their microbes are not in sync, the results can be disastrous

September 06, 2020

Sheng-Yang He’s lab has discovered that plants can develop dysbiosis, a condition similar to inflammatory bowel disease in humans, with severe consequences.

A zest for synthetic biology
Feature

A zest for synthetic biology

September 02, 2020

Metabolic engineers seek to overcome the challenges of mass-producing commodity chemicals, such as limonene, an oil from orange peels.