Annual Meeting

Lipoprotein researcher to talk about structure studies

Melchior refined a technique to separate HDL particles
Courtney Chandler
March 01, 2019

For John Melchior, the thrill of research comes from standing on the precipice of the unknown.

“There is nothing quite like the feeling of striking gold and getting a new piece of critical data,” he said. “At that very moment, you’re likely the only person in the entire world that knows that biology.”

John MelchiorJohn Melchior refined a technique to separate high-density lipoprotein particles based on the presence of certain proteins.

Melchior researches the structural complexity of high density lipoproteins, or HDL, as a postdoctoral fellow in Sean Davidson’s lab at the University of Cincinnati. Based on his work, he was selected by the Journal of Lipid Research to speak in a special session highlighting up-and-coming researchers at the American Society for Biochemistry and Molecular Biology annual meeting.

Melchior said he “fell in love with lipoproteins” during his graduate studies at Wake Forest University, where he was trained in lipid biochemistry. He has continued to study lipoproteins, this time from the protein side. He refined a technique to separate HDL particles based on whether they contain a protein called apoA-II and determined that apoA-II is critical for the positive effects of HDL.

“I found this extremely exciting,” he said. “The logical next steps were to understand the role of apoA-II’s structure in modulating this function.”

His initial attempts to understand the structure of apoA-II were halted when Nature News reported that a previously published crystal structure of the protein was falsified. But Melchior sees what he called an “unfortunate setback” as an opportunity for his structural studies to help fill the gaps of what remains unknown about apoA-II.

Melchior hopes his work will benefit the HDL research community as a whole; for him, this community is almost as important as the research itself.

“I’ve been fortunate to establish relationships with some amazing people who also happen to be excellent scientists,” he said. “I’m really grateful for the community and the friendships that have enriched my life.”

Dissecting the structure of HDL

High density lipoproteins, or HDL, are known as the “good” cholesterol and are thought to decrease risk of heart disease as well as chronic inflammatory diseases. Though commonly thought of as a single number on a patient’s medical chart, HDL is incredibly complex.

The focus of John Melchior’s research is this complexity. He is developing new ways of physically separating subspecies of HDL. Despite their physical similarity, Melchior has been able to isolate very specific subspecies and has found that particles that contain a protein called apoA-II are better at removing cholesterol from cells compared to particles that lack apoA-II. This ability to remove “bad” cholesterol, called the cholesterol efflux property, has been linked to protection against heart disease in several clinical studies.

Melchior’s work could have a direct impact on medical care in addition to contributing to the field of lipid research. The information he gains from understanding the structures of different HDL particles could be used to develop clinical assays that may better identify patients that are at higher risk for heart disease.

Courtney Chandler

Courtney Chandler is a postdoctoral researcher at Johns Hopkins University and an industry careers columnist for ASBMB Today.

Join the ASBMB Today mailing list

Sign up to get updates on articles, interviews and events.

Latest in People

People highlights or most popular articles

Protein society awards; Parise named dean; remembering Bud Patterson
Member News

Protein society awards; Parise named dean; remembering Bud Patterson

March 30, 2020

Awards, honors, promotions and more. Find out what's going on in the lives of ASBMB members.

Stanley Cohen (1922 – 2020)
Retrospective

Stanley Cohen (1922 – 2020)

March 23, 2020

Laura Furge remembers a Nobel laureate who discovered epidermal growth factor and its receptor, work that has allowed generations of biochemists to study the pathways that allow cells to respond to external events.

Chu wins educator award; Cech honored; remembering Holmgren
Member News

Chu wins educator award; Cech honored; remembering Holmgren

March 23, 2020

Awards, promotions and more. Find out what's going on in the lives of ASBMB members.

The best of two worlds
Journal News

The best of two worlds

March 19, 2020

Cecil Pickett’s research career spanned several decades and led to pivotal findings on oxidative stress responses and key breakthroughs in drug discovery.

Ron Kaback (1936 – 2019)
Retrospective

Ron Kaback (1936 – 2019)

March 16, 2020

Gary Rudnick remembers his friend and mentor, a biochemist who combined a deep love of science with a prankish sense of humor.

Patton–Vogt to lead department; Wiley Prize for Rosen; and more
Member News

Patton–Vogt to lead department; Wiley Prize for Rosen; and more

March 16, 2020

Awards, promotions and more. Find out what's going on in the lives of ASBMB members.