Serine proteases in pericellular proteolysis and signaling

Nov. 2–3, 2023

The 2023 virtual meeting on serine proteases in pericellular proteolysis and signaling continues the tradition of the ASBMB special symposium on membrane-anchored serine proteases with the expanded focus on other related serine proteases that function in the pericellular environment.

The conference traditionally brings together leading researchers from across the globe in the fields of serine proteases and pericellular proteolysis, providing them with a forum to present their latest findings, exchange ideas, demonstrate novel technologies and network to form collaborations.

A major foundation of the conference is providing a comfortable venue for junior investigators at the graduate student and postdoctoral level to discuss their current research, have opportunities to meet with experts in the field and forge new scientific interactions crucial to their career development. We are planning an interactive poster session for Q&A with open access to poster presenters' video recordings throughout the conference to increase the visibility of their work.

With the success of the last meeting, we will continue to meet virtually so that the conference is accessible to students and investigators from around the world and to those from other fields where pericellular proteolysis is implicated.

Areas of focus

Cleavage of proteins in the extracellular environment by proteases, whether anchored to the membrane or tethered by cofactors, plays a key role in the regulation of cell behavior under normal and pathophysiological conditions. Among more than 500 proteolytic enzymes encoded by mammalian genomes, membrane-anchored serine proteases, which are expressed on the cell surface in all major organs, are excellently suited to mediate signal transduction across the plasma membrane and are increasingly being recognized as important regulators of organ development and homeostasis. Equally, unrestrained proteolysis and dysregulation of proteolytic cascades, such the complement system, have been shown to contribute to cell barrier dysfunction, inflammatory, cardiovascular, renal, and respiratory diseases, as well as the progression of cancer. Increasingly, proteases and their cofactors have become attractive therapeutic targets, or the pathological protease activity has been capitalized on for conditionally activated therapeutics.

In addition to the roles of serine proteases in physiological and pathophysiological cellular regulation, the meeting will broadly cover topics including:

  • Biosynthesis
  • Trafficking and post-translational modifications
  • Endogenous and pharmacological inhibitors
  • Developmental and other physiological functions
  • Mechanisms of dysregulation and pathological consequences
  • Molecular mechanisms of protease-mediated signaling
  • Novel technologies for better understanding the mechanisms of proteases

Important dates

Sept. 7 Early registration deadline*
Sept. 7 Abstract submission deadline for oral and poster (authors are required to register for the conference upon submission of abstract)
Sept. 25 Late-breaking abstract submission deadline for poster only (authors are required to register for the conference upon submission of abstract)
Sept. 25 Deadline for cancelations/refunds (no refunds after this date)
Oct. 31 Regular registration deadline*

The ASBMB will send abstract programming notifications to oral and poster presenters including access to the virtual platform and upload instructions on Monday, Sept. 18.


Grant E. Blouse Stealth Startup
Anthony O’Donoghue University of California, San Diego


Matthew Bogyo

Matthew Bogyo
Stanford University

Liangliang Hao

Liangliang Hao
Boston University

James Janetka

James Janetka
Washington University in St. Louis

Paulina Kasperkiewicz

Paulina Kasperkiewicz
Wrocław University of Science and Technology

Karin List

Karin List
Wayne State University

Lorraine Martin

Lorraine Martin
Queen’s University Belfast

Sonya Neal

Sonya Neal
University of California, San Diego

Joann Trejo

Joann Trejo
University of California, San Diego

Steven Verhelst

Steven Verhelst
KU Leuven – University of Leuven

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Program schedule

Thursday November 2
Friday November 3

Thursday agenda

9:45 AM - 12:00 PM

Session I

Protease-activated receptor-1 biased signaling
Joann Trejo, University of California, San Diego
Developing precision cancer solutions via microenvironmental protease profiling
Liangliang Hao, Boston University
An in vitro platform enables catalytic characterization of an intramembrane rhomboid protease Rhbdl4
Sonya Neal, University of California, San Diego
Engineering cellular responses to pericellular proteolysis with synthetic protease-activated receptors
Matthew Ravalin, Stanford University School of Medicine
Targeting protease driven pathways in FSGS
Priyanka Rashmi, University of California, San Francisco
12:00 PM - 1:00 PM


1:00 PM - 3:30 PM

Session II

Mechanistic studies of inhibitory anti-tryptase antibodies for the treatment of asthma
Henry Maun, Genentech Inc.
Dual inhibitors of matriptase and TMPRSS2 as broad-spectrum antivirals
James Janetka, Washington University in St. Louis
Spike protein interactions with SARS-CoV-2 receptors at intestine mucosal surfaces
Klaudia Brix, Constructor University
Furin and other proprotein convertases differently activate the human papillomavirus (HPV)
Gonzalo Izaguirre, University of Illinois Chicago
Flash talk: Structural and mechanistic basis for the maturation of site-1 protease in the secretory pathway
Daniel Kober, University of Texas Southwestern Medical Center
Flash talk: Identification of host cell surface proteases inhibitors as antivirals against respiratory viruses
Gabriel Lemieux, Université de Sherbrooke
Flash talk: Targeting SERPINs as a therapeutic strategy in ameliorating neurodegenerative disease
Matthew Avalos, Baylor College of Medicine
Flash talk: Inhibiting human neutrophil serine protease 4 with mRNA display-derived macrocyclic peptides
Wanjian Tang, Genentech Inc.
Flash talk: Bioprospecting for serine protease inhibitor activity in insect venoms
Nathan Mortimer, Oregon State University
3:00 PM - 3:30 PM


3:30 PM - 4:00 PM

Poster session

4:00 PM - 5:00 PM

Social hour

Friday agenda

9:45 AM - 12:00 PM

Session III

Putting the CAP on ENaC: The role of serine proteases in the regulation of airways hydration and mucociliary clearance in chronic airways diseases
Lorraine Martin, Queen's University Belfast
Development and application of quenched activity-based probes for imaging of pericellular serine proteases
Steven Verhelst, KU Leuven – University of Leuven
Heterogeneity of neutrophils based on serine proteases
Paulina Kasperkiewicz, Wrocław University of Science and Technology
HAT/DESC proteases and their role in epithelial homeostasis in the oral cavity
Carly Martin, National Institute of Dental and Craniofacial Research
Potent and selective cystine-knot peptide inhibitors of the serine protease HTRA1 bind to a cryptic pocket within the active site region
Daniel Kirchhofer, Genentech Inc.
12:00 PM - 1:00 PM


1:00 PM - 3:30 PM

Session IV

Identification of proteases from commensal bacteria that regulate PAR signaling in the gut
Matthew Bogyo, Stanford University
Cancer promoting and suppressing properties of cell-surface anchored serine proteases
Karin List, Wayne State University
DIANA: A novel method for ultrasensitive FAP quantification and high-throughput screening in drug development
Katerina Cermakova, Institute of Organic Chemistry and Biochemistry of the CAS
Transmembrane serine protease TMPRSS11B promotes tumor progression in lung squamous cell carcinoma through enhanced lactate export and modulation of the tumor microenvironment
Hari Shankar Sunil, University of Texas Southwestern Medical Center
Flash talk: Structure-guided identification of conformationally selective inhibitory antibodies to serine proteases
Kyle Anderson, University of California, San Francisco
Flash talk: The impact of chymase in colonic inflammation: Proteolytic cleavage of epithelial integrity-related proteins
Afshin Derakhshani, University of Calgary
Flash talk: Epithelial barrier integrity and transepithelial sodium transport are regulated in tight epithelia by several membrane-bound serine proteases
Edith Hummler, University of Lausanne
Flash talk: Characterizing the metastasis-suppressive function of the cell-surface protease prostasin in breast cancer
Joseph Lundgren, Wayne State University
Flash talk: Critical role of fibrinolysis in hematopoietic recovery after myelosuppression
Tiffany Nguyen, National Institute of Dental and Craniofacial Research
3:00 PM - 3:30 PM


3:30 PM - 4:00 PM

Poster session

4:00 PM - 5:00 PM

Business meeting, closing activities & poster prizes