January 2012

W. Neal Burnette: the man behind the Western blot

Burnette made his seminal contribution to molecular biology and biochemistry as a postdoc and went on to have an unusual career.


W. Neal Burnette retired in 2005 as a colonel in the U.S. Army after serving 35 years in various positions. Photos courtesy of Burnette.

Thirty-one years ago, W. Neal Burnette published a paper that described a technique called Western blotting 1). The paper initially was rejected by the journal Analytical Biochemistry, but it went viral among molecular biologists as a preprint. Eventually the journal agreed to publish the paper, which now has been cited more than 6,000 times. Because Burnette didn’t bestow his name on the blot, it’s likely that the current generation of investigators don’t know he was involved in developing the technique that is now ubiquitous in molecular biology and biochemistry research laboratories and used as a clinical diagnostic for HIV-AIDS.

Burnette’s story is an unusual one: After a quick dip into acting school after high school, he went through the academic training mill. In the early 1980s, he went to work for a then-small biotechnology company. At the same time, Burnette served his country for 35 years, in both reserve and active duty, as a U.S. Army officer, a field medic and an infectious diseases expert.

Early years
Born in 1944 in New York state, Burnette traveled with his family all over the U.S. and Japan while his father served in the U.S. Air Force. Burnette wanted to be a military pilot like his father, who was a bomber pilot during World War II and a fighter pilot during the Korean war, but his bad eyesight put an end to that dream. He was very much interested in science, but, in his junior year of high school, his mother encouraged him to take speech and drama classes after he got injured during football spring training. Burnette says he became “a starry-eyed kid who wanted to be an actor.”

As a senior in a Texas high school, Burnette acted in the lead role of King Creon in a production of Jean Anouilh’s “Antigone,” which won him and his school theater awards from the state. The award led to a theater scholarship from Texas Christian University. “I went to college to study theater and ballet,” he says. “But my then-girlfriend, and now wife, convinced me to change course.” Aware of Burnette’s interest in science, she told Burnette she would marry him only if he went into science.

Burnette heeded her. “I lost my theater scholarship. I worked 48 hours a week as a surgical scrub” to make ends meet, recalls Burnette. “It was tough getting through school.”

With mentorship from his organic chemistry professor, Manfred Reinecke, Burnette graduated with two bachelor’s degrees, one in biology and the other in chemistry. He then got a master’s degree in bio-organic chemistry at the University of Central Missouri, a location chosen partly because his father was teaching there. He then went to Vanderbilt University to work toward a Ph.D. thesis on RNA tumor viruses (now called retroviruses) under the guidance of William Mitchell. This was followed by a postdoctoral stint with J. Thomas August, who was at that time at the Albert Einstein College of Medicine, where Burnette honed his skills in SDS-PAGE and radioimmunoassays.


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Nice article. So, is it "Western" or "western"? Or does either work, without a preference? - Bruce Maryanoff (bmaryano@scripps.edu)


I was a high school friend of Neil's and recall recognizing how committed he was to acting as we enjoyed a coke one evening at local hangout and he still had part of his make up on from a dress rehersal he had just attended. Little did I know how focused he would become on a wide range of endeavors or how greatly he would apply himself to so very many. I next met up with him at a 40th reunion and was thoroughly impressed by the reach of his shift to scientific discipline. At the time he was a full colonel filling a general's slot in the US Army. Congratulations to Neil on a life well lived and leaving a trail of useful accomplishments. And thanks to the author for seeing that such a life does not escape notice. James Sizemore - Bedford, TX.


I went to high school with Neil and we were good friends. After going our separate ways I lost touch with him. Now that we are retired I located him via facebook, and retrieved this article via that link. I used the western blot method while I was teaching and never knew it had a connection to an old buddy. I find the article fascinating, well written and a worthy tribute to someone who has so effectively contributed to scientific knowledge. I wish to thank the author for giving credit where credit was obviously due. A Cheer for Neil! With best regards, Gil R Alexander, Retired Director of the Montana Science Institute, and former President of the Montana Academy of Sciences



  • Neal: I just received an e-mail from Jim Kaper in which he said he and his wife had just returned from India with a visit to the Taj Mahal where he and I bumped into each other in 1990 by accident (we were there for different meetings). I was thus responding to him that you and I had visited there in 1992 and had our picture taken in front of the same minaret, except my picture with him was from 15 feet away since the place was crowded with 1000s of people whereas our picture was taken from over 100 feet away since we visited during a curfew where we had the whole place to ourselves with nobody but us and our guides! The two pictures are still on my bulletin board in my office to remind me of happy days and good friends. Hope all is well. Best regards, Roy

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