Glycolipids play diverse biological roles — from serving as receptors of toxins and growth factors to overseeing molecular recognition at the cell surface. These molecules are composed of a ceramide backbone to which monosaccharide (sugar) molecules are attached. The order in which different sugars are attached to the ceramide backbone is a crucial feature in determining the precise role of a glycolipid. Understanding the machinery that regulates the glycosylation of the ceramide backbone, therefore, is critical to our understanding of the biosynthesis of these important lipids and provides insight into the mechanisms underlying diseases resulting from aberrations of their synthesis. Interestingly, the organization of the glycosylation machinery within the Golgi is linked intimately to the supramolecular organization and dynamics of the Golgi complex itself.