November 2010

[MCP] Analyzing Cyano­bacterial Stress

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MCP

Global stress response of different cyanobacterial pathways; colors indicate either increased (red) or decreased (green) protein abundances under stress compared to control; black indicates that proteins are not observed in control sample.

Cyanobacteria are the only prokaryotes capable of oxygenic photosynthesis and thus play crucial roles in global carbon and nitrogen cycles. Cyanobacteria are present in a wide range of ecological niches and have developed a host of stress responses to accommodate changes in the environment. To gain a more detailed knowledge of these responses, the researchers in this study performed a large-scale proteomic analysis of the model cyanobacterium Synechocystis sp. PCC 6803 using 33 different environmental conditions (altered temperatures, nutrient depletion, etc.). They identified over 22,000 unique peptides corresponding to 1,955 proteins (covering 53 percent of the predicted proteome), of which 1,198 were differentially regulated in response to environmental stresses. Notably, they found that various perturbations resulted in the activation of atypical pathways for the acquisition of carbon and nitrogen from urea and arginine, suggesting that this could be a common stress response. This study provides the most comprehensive functional and quantitative catalog of the Synechocystis proteome to date and will be quite valuable for future experimental studies for this important group of organisms.

Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium under Diverse Environmental Perturbations

Kimberly M. Wegener, Abhay K. Singh, Jon M. Jacobs, Thanura R. Elvitigala, Eric A. Welsh, Nir Keren, Marina A. Gritsenko, Bijoy K. Ghosh, David G. Camp II, Richard D. Smith and Himadri B. Pakrasi

Mol. Cell. Proteomics, published online Sept. 21, 2010

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