April 2010

Symposium: Post-translational Modifications: Detection and Physiological Evaluation



With the completion of the human genome and rapid progress in understanding the proteome, it now is clear that the next phase of molecular bioscience largely will, by necessity, focus on post-translational modifications (PTMs) and their regulation of protein functions. There are more than 400 known PTMs, and, almost without exception, virtually all polypeptides are post-translationally modified. Given the relatively small number of genes and polypeptides in complex organisms, PTMs create the enormous and highly dynamic structural and functional diversity required to regulate essential cellular functions. In recent years, the technologies used to quantitatively study PTMs at the molecular and structural level have developed rapidly, particularly in the area of mass spectrometry. This biannual meeting brings together leading experts in the study of a wide variety of different PTMs to allow cross-fertilization, presentation of the most exciting breakthroughs in the methodology and biological functions of PTMs and lively discussions of new concepts and approaches.

Gerald W. Hart

Most of the talks will be selected from abstracts submitted by participants, allowing discussion of the most recent and exciting developments within the field. However, there are several scheduled presentations:

• Tony Hunter of the Salk Institute will present the keynote lecture on the role of protein phosphorylation and other PTMs in the regulation of cell growth and cell division.

• Alma Burlingame of the University of California, San Francisco, will describe his work on the development of state-of-the-art methodologies in mass spectrometry to study O-GlcNAc modifications and the GlcNAc/phosphorylation interplay.

• Brent Martin of the Scripps Research Institute will discuss novel approaches for large-scale analyses of protein palmitoylation in mammalian cells.

• Robert Chalkley of the University of California, San Francisco, will describe innovative developments using database searches for analysis of PTMs.

Post-translational Modifications: Detection and Physiological Evaluation
Oct. 21 – 24, 2010
Granlibakken, Lake Tahoe, Calif.
Short talk and poster abstract submission deadline: Sept. 15, 2010
Early registration deadline: Aug. 15, 2010

• Richard D. Cummings of the Emory University School of Medicine will present his pioneering work on the roles of complex protein-bound glycans in cell adhesion, development and microbial or viral pathogenesis.

• Donald F. Hunt of the University of Virginia will discuss his work on the development of new methods and instrumentation for the characterization of post-translational modifications and their application to epigenetics.

• Stuart A. Lipton of the Burnham Institute for Medical Research will present his work on the molecular mechanisms of neurodegenerative diseases and stroke, including his discovery of the roles of protein S-nitrosylation.

• Kevin L. Moore of the Oklahoma Medical Research Foundation will describe his novel work on the enzymology, biology and functions of tyrosine sulfation.

• Akhilesh Pandey of the Johns Hopkins University School of Medicine will share his findings and views with respect to PTMs and signaling and recent developments in databases for system analyses of PTM biology.

• Tony Pawson of the Samuel Lunenfeld Research Institute will lecture on mechanisms that underlie intracellular signal transduction by modular-protein domains.

• Pierre Thibault of the Université de Montreal will talk about high field asymmetric waveform ion mobility spectrometry (FAIMS) and phosphorylation and about his recent work on protein sumoylation.

Based on the enormous success of the first meeting held two years ago in Lake Tahoe, this meeting not only will benefit newcomers to the field of PTMs but it also will provide a forum for sharing breakthroughs in both methodology and biology for established investigators.

Katalin Medzihradszky (folkl@cgl.ucsf.edu) is a professional research chemist at the University of California, San Francisco, and Gerald W. Hart (gwhart@jhmi.edu) is an investigator at Johns Hopkins University.

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