March 2012

The men behind Western blotting

  1. 1) Mukhopadhyay, R. W. Neal Burnette: the man behind the Western blot. ASBMB Today, January 2012:17 – 19.
  2. 2) Renart, J.; Reiser, J.; and Stark, G. Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proc. Natl. Acad. Sci. USA. 1979; 76:3116 – 3120.
  3. 3) Alwine, J.C.; Kemp, D.J.; and Stark, G. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc. Natl. Acad. Sci. USA. 1977;74:5350 – 5354.
  4. 4) Southern, E.M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 1975;98:503 – 517.
  5. 5) Noyes, B.E., and Stark, G. Nucleic acid hybridization using DNA covalently coupled to cellulose. Cell, 1975; Volume 5:301 – 310.
  6. 6) Towbin, H. Origins of protein blotting Biji T. Kurien and R. Hal Scofield (eds.), Methods in Molecular Biology, Protein Blotting and Detection, vol. 536 Humana Press, a part of Springer Science & Business Media, LLC, 2009.
  7. 7) Towbin, H.; Staehelin, T.; and Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 1979;76:4350 – 4354.
  8. 8) Burnette, W.N. “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 1981;112:195 – 203.
  9. 9) Eisenstein, M. A look back: westward expansion. Nature Meth. 2005;2:796.
  10. 10) Mukhopadhyay, R. Revamping the Western blot. ASBMB Today. January 2012:14 – 16.

Raj_MukhopadhyayRajendrani Mukhopadhyay ( is the senior science writer for ASBMB Today and the technical editor for the JBC.

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Note to the author: when referring to RNA or DNA blotting, it should not be called immunoblotting since doesn't rely on the use of antibodies to detect molecules as in western blotting. Editor's note: Thank you. The article has been corrected.



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