December 2011

Thousands of expression plasmids and many empty vectors available

online_plasmid

The  PSI:Biology-Materials Repository recently announced that it now has more than 50,000 protein expression plasmids and almost 100 empty vectors. This includes about 900 membrane protein plasmids, and that number is expected to grow in the coming months.

Established in 2006, the PSI:Biology-Materials Repository provides centralized storage, maintenance and distribution of plasmid clones produced by researchers involved in the Protein Structure Initiative. Each PSI plasmid comes with detailed information about the vector, insert and protein expression. The information is curated by scientists and housed at Arizona State University.

The main ASU repository offers plasmids from hundreds of organisms and special collections, including human kinases, the Thermotoga maritime genome and a new set of 180 glycoenzymes. Each plasmid has its sequence verified and gets stored in a state-of-the-art automated freezer storage system. The repository also is connected to the PSI-Nature Structural Biology Knowledgebase, which cross-references a particular plasmid to protein annotations and experimental data. Researchers can search for and request plasmid samples for a modest fee.


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