December 2010

Retrospective: Bernard L. Horecker

ASBMB Past-president Bernard Leonard Horecker died this past October. He was well know for his contributions to elucidating the pentose phosphate pathway.


Bernard L. HoreckerBernard Leonard Horecker, best known for his contributions to elucidating the pentose phosphate pathway, died on Oct. 9, 2010. He was president of the American Society for Biochemistry and Molecular Biology in 1968.

Horecker was born in Chicago in 1914. He began his training in enzymology in 1936 as a graduate student at the University of Chicago in the laboratory of T. R. Hogness, searching for an enzyme that would catalyze the reduction of cytochrome c by reduced NADP. This marked the beginning of his lifelong involvement with the pentose phosphate pathway.

After earning his doctoral degree, Horecker got a job at the National Institutes of Health in Frederick S. Brackett’s laboratory in the Division of Industrial Hygiene. As part of the war effort, he was assigned the task of developing a method to determine the carbon monoxide hemoglobin content of the blood of Navy pilots returning from combat missions.

When the war ended, Horecker remained at the NIH and returned to research in enzymology. He began to study the reduction of cytochrome c by the succinic dehydrogenase system. This work led to a collaboration with Arthur Kornberg in which the two studied the effects of cyanide on the succinic dehydrogenase system. Cyanide previously had been found to inhibit enzymes containing a heme group, with the exception of cytochrome c. However, Horecker and Kornberg discovered that cyanide did, in fact, react with cytochrome c and concluded that previous groups had failed to perceive this interaction because the shift in the absorption maximum was too small to be detected by visual examination.

Two years later, Kornberg invited Horecker and Leon Heppel to join him in setting up the new Section on Enzymes in the Laboratory of Physiology at the NIH. Their section eventually became part of the new Experimental Biology and Medicine Institute and was later renamed the National Institute of Arthritis and Metabolic Diseases.

Horecker and Kornberg collaborated again, this time on the isolation of NAD (DPN) and NADP (TPN). By 1948, they had amassed a substantial supply of these materials and were able to present Otto Warburg, the discoverer of NADP, with a gift of 25 mg of the coenzyme when he visited the NIH. Horecker also collaborated with Heppel on the isolation of xanthine oxidase from milk, which unexpectedly reduced cytochrome c only in the presence of oxygen, an observation that eventually led to a widely used assay for the detection of the superoxide anion.

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I first met Bernie 53 years ago, in 1958 in Vienna, at an Annual Reviews of Biochemistry Editors meeting during the IUB Congress. Therafter we became close friens and collaborators as editors and in Society affars.As the ASBC (now ASBMB) President he was enormously helpful to me in founding the Pan-American Association of Biochemists. He would give freely of his energy to any cause he considered worthwhile.I last saw him at a FASEB Summer Conference in Colorado in 1987.Soon thereafter he retired to the west coast of Florida, which, I thought, would provide more opportunities to meet. Not so, the best example being in 1992 at the Miami Winter Symposium, where two of his oldest friends, Arthur Kornberg and Severo Ochoa took part. I tried hard to persuade Bernie to join us, but to no avail.Our last communication was the next year, when Severo and my wife died within a short time of each other. On learning of his death I looked him up in the FASEB Directory. He had kept up his membership and included an e

 

Dr. Horecker (I could never call him Bernie) was the reason I left medical school to become his first graduate student. This was a personal triumph for him, and a wonderful opportunity for me. I owe so much to his guidance and example, and to the great group of post-doctoral fellows and foreign visitors in his lab that were a resource for this very novice biochemist. Thanks to his close association with Monod at the Pasteur, he was able to arrange for me to spend my post-doc in Paris. It was there that I learned bacterial genetics, the lac operon, etc., and to decipher the notes that Francois Jacob produced for me to study after our conversations. We lost touch after I returned to the US, but I have always had his voice in my head guiding my interactions with graduate students, and trying to do biochemistry. I will never forget him, and the debt that I owe him Michael Malamy, Tufts University

 

This is a fine summary of his achievements and the excellence and rigor or his science, but it doesn't capture the dapper and supportive Bernie, that the students and postdocs in his departments at NYU and Einstein will hold fondly in their memories. Ann Skalka, Ph.D. Fox Chase Cancer Center.

 

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