MCP to spotlight systems biology
and proteomics

Published April 01 2018

Molecular & Cellular Proteomics will sponsor a session on systems biology and proteomics at the 2018 American Society for Biochemistry and Molecular Biology Annual Meeting. Leading researchers in the field will discuss state-of-the-art systems biology and proteomic techniques applied in a number of areas, including virus infection, biomarker discovery and validation, and drug response.

The session will begin at 10 a.m. April 23 in Room 6E of the San Diego Convention Center.

10 A.M.

Protein localization and interaction partners

Knowing where a protein resides within the cell and what other proteins it interacts with is crucial to understanding its molecular functions. MCP Deputy Editor Steven Carr of the Broad Institute will present new approaches for studying subcellular localization of proteins and their interaction partners with high spatial resolution. Carr will describe the proximity labeling technique, where the protein of interest is fused to a promiscuous biotin ligase to enable the identification of its interacting partners using mass spectrometry. His talk will focus on how the technique has been used to investigate fundamental biological questions, such as mapping mitochondrial matrix proteins and defining the proteomes of synapses of living neurons. He also will describe new work on developing genomic locus proteomics, a technique that combines proximity labeling, genome targeting and quantitative mass spectrometry to identify proteins that occupy specific genomic loci.

Steve Carr

10:30 A.M.

Building an operating system for cancer

With systems biology comes big data. A challenge to advancing precision medicine to treat cancer is how to integrate massive data sets and studies to translate to actionable information for patients. Kevin White of the University of Chicago will talk about building an operating system for cancer. He will discuss the steps that have been taken to develop technological solutions and novel approaches to the challenge of integrating massive data sets and validating discoveries. He will describe his efforts to engineer and industrialize a systematic solution to allow physicians to treat patients using a real-time machine learning system that condenses petabytes of data into actionable information matched uniquely to each patient.

Kevin White

11:00 A.M.

Virion display technology to characterize GPCRs

G-protein coupled receptors, or GPCRs, are a family of membrane-bound receptors that are notoriously difficult to study because they must be embedded in a membrane to maintain native conformation. Heng Zhu of the Johns Hopkins University School of Medicine will describe the development of a virion display array technology, VirD, where GPCRs are displayed in the membrane envelope of virions to maintain their native conformation. Zhu, who is also an MCP editorial board member, will discuss how the VirD approach has been used to characterize orientation, ligand-binding activities and protein targets of the GPCRs. He plans to illustrate the utility of VirD technology as a high-throughput platform for screening of drugs, affinity reagents, ligands and more.

Heng Zhu

11:30 A.M.

Proteome organization during viral infections

Viruses remodel host cellular organelles, such as mitochondria, to suppress host defense and promote viral replication. Session organizer Ileana Cristea of Princeton University will give a talk on how her laboratory integrates experimental studies, mathematical modeling and inference methods to study the proteome dynamics of host cells during viral infections. Cristea, an MCP editorial board member, will describe how methods such as live-cell microscopy and mass spectrometry-based proteomics have been used to discover the dynamic changes that occur in organelle composition and subcellular localization in response to viral infection. She will highlight how integrative -omic technologies can help paint a picture of the pathogen’s life cycle and aid in the future development of antiviral therapies.

Ileana Cristea
Saddiq Zahari Saddiq Zahari is the editor for manuscript integrity at MCP.