UBC researchers identify gene in balsam fir that could facilitate cheaper and more sustainable production of fixatives and scents used in the fragrance industry
April 9, 2012 — University of British Columbia researchers have identified a gene in balsam fir trees that could facilitate cheaper and more sustainable production of plant-based fixatives and scents used in the fragrance industry and reduce the need for ambergris, a substance harvested from whale barf.
Bifunctional cis-Abienol Synthase from Abies balsamea Discovered by Transcriptome Sequencing and Its Implications for Diterpenoid Fragrance Production
Background: Balsam fir produces cis-abienol, a natural product of value to the fragrance industry.
Results: We describe the genomics-based discovery of balsam fir cis-abienol synthase.
Conclusion: cis-Abienol synthase is a bifunctional diterpene synthase that produces a bicyclic diterpenol in the class II active site.
Significance: cis-Abienol synthase is a new enzyme for metabolic engineering of plants or microorganisms to produce high value fragrance compounds.
When sperm whales consume sharp objects, such as seashells and fish bones, their gut produces a sticky substance to protect their digestive organs. They then regurgitate the mixture — much like cats throwing up fur balls — and the vomit, reacting with seawater, turns into rock-like objects that wash ashore. These are collected and refined for their fixative properties. Called ambergris, the scented compound is added to high-end perfumes to help the fragrance stay on the skin longer.
The discovery was led by Prof. Joerg Bohlmann and postdoctoral research associate Philipp Zerbe at UBC’s Michael Smith Laboratories. Details are published in the April 6 issue of The Journal of Biological Chemistry.
“The use of ambergris in the fragrance industry has been controversial,” says Bohlmann, who is a professor of Botany and Forest Sciences. “First of all, it’s an animal byproduct and the use of such in cosmetics has been problematic, not to mention it comes from the sperm whale, an endangered species.”
Even though much of the ambergris approved for use today is manually collected along the shorelines of known sperm whale habitats in the Atlantic and Pacific Oceans and in the Caribbean, it is still a costly venture. In the Mediterranean, sage has been cultivated for the production of a plant-based substitute of ambergris, but yields are variable and can be unpredictable, similar to manual collection of ambergris.
“We’ve now discovered that a gene from balsam fir is much more efficient at producing such natural compounds, which could make production of this bio-product less expensive and more sustainable,” says Bohlmann.
The discovery and related technology is currently being commercialized through UBC’s University-Industry Liaison Office. The research was supported by Genome Canada, Genome British Columbia, and Genome Alberta through the PhytoMetaSyn Project, and through grants from the Natural Sciences and Engineering Research Council of Canada.
Press release courtesy of University of British Columbia Public Affairs. You can find the original press release here.
Photo courtesy of the Zerbe et al article in JBC.