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Cloning jobs

8/31/2015 12:56:14 PM

Going back to the basics and perfecting your biochemistry and molecular biology techniques is always a great way to open yourself to research jobs in a variety of fields, and one of the most essential basic techniques to master is cloning. Can you think of any other basic techniques that are so essential to a research scientist’s toolkit? Being able to manipulate PCR products and plasmids to generate constructs for your experiments may seem easy, but it can be surprisingly intricate. Companies that make reagents always are coming up with new tools to aid this process, so staying on top of the latest and greatest trends is also important. Along with cloning, knowing how to properly design PCR primers to facilitate your cloning or how to select restriction enzymes to use are also essential. This week, I found a great variety of jobs in the health science, plant science and microbial engineering fields, and all of them want scientists who know how to clone!

Open positions

Intrexon (San Francisco) seeks a next-generation sequencing scientist to join its team and lead research-and-development efforts to validate plasmids and perform other sequencing tasks, including whole-genome and RNA-seq experiments. The incumbent will oversee operation of MiSeq sequencing at the division and perform method-development tasks, including scale-down, sample-prep optimization and data quality optimization, as well as assist in running computational pipelines and other laboratory tasks as needed. Requires a Ph.D., M.S. or equivalent experience in molecular biology or a related discipline. Strengths in cloning and other molecular biology techniques are a plus, as are skills in Python, Perl or related programming languages.

AbbVie (Worcester, Mass.) seeks a senior scientist II in cellular and molecular biology to work on its External Alliances and Sequencing Team. Candidates should have experience in next-generation sequencing, automation and Sanger sequencing. Support sample preparation and data analysis, and develop high-throughput workflows for hybridoma and/or B cell cloning, as well as hybridoma cDNA cloning. Requires a Ph.D., M.S. or B.S. degree in chemistry, biochemistry, biophysics or a related field, plus experience.

Cargill (Minnetonka, Minn.) seeks a research biotechnologist for a two-year assignment. Generate improved microbial strains, which requires the ability to independently design, synthesize and assemble genetic constructs and accurately integrate these into microbial strains as well as through mutagenesis and selection. Complete strain evaluations by DNA sequence and structure analysis, RNA expression analysis and enzyme assays. Develop and optimize methods for strain construction and evaluation, evaluate strains for performance characteristics at a range of small scales (microplate to shake flask). Collect, organize and interpret data and develop next steps, report results in written form and provide summaries of work and interpretation of results to the project group in presentations. Requires a Ph.D., master’s or bachelor’s degree with an emphasis in a biological sciences discipline, as well as hands-on lab experience in molecular biology, gene cloning and assembly, PCR and metabolic engineering.

Trans Ova Genetics (Sioux Center, Iowa) seeks a junior research scientist to plan and carry out experiments. Establish cell lines from fetal recoveries, calf ear biopsies and embryos; extract DNA and RNA from these cell lines and tissue samples; and set up PCR-based assays to detect specific genes and biomarkers. Work with research technicians and other available support from the IFV and cloning production laboratories. Requires an M.S. in animal reproduction or a related field with a strong background in molecular techniques and laboratory experience.

Benson Hill Biosystems (St. Louis) seeks a senior research associate in plant physiology to perform experiments designed to assess photosynthetic efficiency and plant primary metabolism in an extremely fast-moving biotech startup. Requires familiarity or experience with standard molecular biology and biochemistry protocols (e.g., cloning, PCR, RT-PCR, enzyme assays, etc.) and basic bioinformatics tools, as well as an M.S. in plant physiology, biotechnology, agronomy or a related plant science discipline.

University of Texas Health Science Center (San Antonio, Texas) seeks a research associate for an enzymology lab primarily investigating the mechanisms of action of flavoproteisn and metalloproteins. Conduct experiments using methods such as protein purification, cloning and mutagenesis, liquid chromatography, electrophoresis and enzyme kinetics. Develop new techniques and procedures to enable experiments and projects to be carried out efficiently and data to be collected effectively. Requires a bachelor’s degree in a specialized field of study with four years of experience, and a master’s degree is preferred.

Rutgers University (Newark, N.J.) seeks to fill a laboratory research IV position in its FASN–Biological Sciences department. Perform entry-level experiments using standard, basic laboratory techniques, keep records of work performed and collect, sort and process samples. Train and lead students through various laboratory-related activities, coordinate and schedule students’ lab work shifts, and provide technical and administrative support. Requires a strong background in molecular and cellular biology, particularly molecular cloning, culturing cell lines or culturing primary rodent cells or tissue organiods.

Washington University in Saint Louis (St. Louis, Mo.) has a research opportunity in the pulmonary department at its medical school. The lab focuses on the mechanisms that regulate T-cell responses in health and disease. The lab is particularly interested in how costimulatory molecules regulate inflammatory diseases in the lung. It uses animal models and human subjects. Requires a master’s-level education with two years of relevant laboratory experience and a knowledge of basic biochemical and molecular biological techniques, including gel electrophoresis, Western blotting, immunoprecipitation, restriction digestion, cloning and more.

Looking for more jobs posts? Check out some of the other posts I’ve written about immunology, lipid research, bioinformatics, translational research, pathology, genomics, developmental biology, biodefense, biochemistry, virology, molecular biology, pharmacology, sequencing, neuroscience, drug discovery, stem cell research, mass spectrometry, plant biology, microbiome, RNA research, metabolism research, microscopy, genetics, protein purification, neurobiology, structural biology, cancer biology, microbiology, and cell culture jobs. Looking for other tips on how to search for jobs? I wrote posts on networking, strategies for job searching (part I and part II), how to decipher a job post, the job acquisition process, and four things to consider when choosing a career. As always, we’d love to hear your feedback or suggestions. Leave a comment or reach out to ASBMB on Twitter or Facebook!